I-Beta-glucans Blocker yokuThintela iBeta Glucan Pathway
I-Beta-glucans Blocker yokuThintela iBeta Glucan Pathway
1. Ulwazi lwemveliso
Kukho iindlela ezimbini kwi-limulus amoebocyte i-lysate lal reagent, i-factor C indlela echanekileyo kwi-endotoxin kunye ne-factor G indlela ethile (1,3) - β-D-Glucans.Ukuba isampuli yovavanyo iqukethe i-β-1,3-Glucans, uvavanyo lwe-limulus (uvavanyo lwe-Endotoxin) luya kuba nokuphazamiseka.I-β-G-Blocker ivimba ukuphinda kusebenze kwe-LAL ukuya kwi-β-1,3-Glucans, inikezela ngokuthe ngqo kwe-endotoxin kuvavanyo lwe-LAL.Ukuba iisampuli zovavanyo ziqulethe iβ-1,3-Glucan, njengeselulosi, usebenzisai-beta-glucans blockerkuya kuba ngumbono olungileyo ukuvimba ukuphazamiseka kwe-β-1,3-Glucan kwi-endotoxin kunye ne-reagent reaction ye-LAL.
2. Ipharamitha yemveliso
Inqanaba le-Endotoxin ngaphantsi kwe-0.005EU/ml
3. Iimpawu zemveliso kunye nesicelo
Indawo ye-LAL ye-Reagent yamanzi ukuze iphinde ihlanganise i-reagent ye-LAL, ivimba i-beta-glucan mediates factor G indlela kwaye iqinisekisa ukuba i-reagent ye-LAL isabela kuphela kwi-endotoxin.Kwiisampuli zovavanyo ezinokungcola (1,3)- β-D-Glucan.
Ikhathalogu No. | Inkcazo | Phawula | Iphakheji |
BH10 | 50mM Tris buffer, pH7.0, 10ml/vial | Isetyenziselwa ukungxenga iisampulu ezineasidi kakhulu okanye ezisisiseko. | Iingqayi ezili-10 / ipakethe |
BH50 | 50mM Tris buffer, pH7.0, 50ml/vial | Isetyenziselwa ukungxenga iisampulu ezineasidi kakhulu okanye ezisisiseko. | Iingqayi ezili-10 / ipakethe |
NGE-10 | 10mM Magnesium Buffer, 10ml/vial | Isetyenziselwa ukoyisa umphumo we-chelation. | Iingqayi ezili-10 / ipakethe |
BT10 | β-glucans blocker, 10ml/vial | Isetyenziselwa ukuvimba ukuphazamiseka kwe-β-glucan kwi-Amebocyte Lysate endotoxin reactions. | Iingqayi ezili-10 / ipakethe |
PBS50 | I-PBS buffer endotoxin-free, 50ml/vial | Sebenzisa ukuhlamba izikhongozeli zeesampuli, okanye uhlengahlengise i-pH | Iingqayi ezili-10 / ipakethe |
PBS500 | I-PBS buffer endotoxin-free, 500ml/vial | 1 ingqayi |
Imeko yeMveliso
Uvakalelo lwe-Lyophilized Amebocyte Lysate kunye namandla okuLawula i-Endotoxin esemgangathweni ivavanywa ngokuchasene ne-USP Reference Standard Endotoxin.Iikiti ze-reagent ye-Lyophilized Amebocyte Lysate ziza nomyalelo wemveliso, iSatifikethi sokuHlalutya.