Endpoint Chromogenic Kit EC80545
IKit yoVavanyo lwe-Bioendo EC Endotoxin (Indawo yokuphela kwe-Chromogenic Assay, iDiazo Coupling)
1. Ulwazi lweMveliso
I-Bioendo EC I-Endotoxin Test Kit (i-End-point Chromogenic Assay, i-Diazo Coupling) ibonelela ngomlinganiselo okhawulezayo we-endotoxin quantification.I-endotoxin kwisampulu ivula i-cascade ye-enzymes kwi-Amebocyte Lysate, i-enzyme esebenzayo yahlula i-substrate yokwenziwa, ikhupha into enombala otyheli.Emva koko into etyheli inokuphinda iphendule kunye ne-diazo reagents ukwenza izinto ezimfusa ezinobuninzi obufunxayo kwi-545nm.I-spectrophotometer eqhelekileyo okanye umfundi we-microplate uyafuneka kwi-assay.Izinto ezimfusa zihambelana nokuxinana kwe-endotoxin.Ke iziphumo zovavanyo lwe-endotoxin luhlalutyo lobungakanani.
2. IParamitha yeMveliso
I-Sensitivity Range: 0.01-0.1EU/ml (ixesha lokuvavanya malunga nemizuzu ye-46)
0.1-1.0EU/ml (ixesha lokuvavanya malunga nemizuzu eyi-16)
3. Iimpawu zeMveliso kunye neSicelo
I-Bioendo EC I-Endotoxin Test Kit (i-End-point Chromogenic Assay, i-Diazo Coupling) yenzelwe ukusetyenziswa kwi-In Vitro ukufumanisa kunye nobungakanani be-gram-negative bacterial endotoxins.Isisombululo esingenambala sokwenziwa kwe-peptide substrate songezwa kwi-Lyophilized Amebocyte Lysate, kwaye emva koko uvavanyo lomzekelo womxube.Ukuba umzekeliso uqulethe i-endotoxin, umbala womxube womzekelo uyatshintsha.I-absorbence inxulumene nokugxininiswa kwe-endotoxin.Ke ngoko amanqanaba e-endotoxn kumxube wesampulu angacaciswa ngokuchasene negophe eliqhelekileyo.I-spectrophotometer eqhelekileyo kunye ne-540 - 545nm isihluzi sanele ukulinganisa i-endotoxin kunye ne-EC Endotoxin Test Kit yethu (I-End-point Chromogenic Assay, i-Diazo Coupling).
Phawula:
I-reagent ye-Lyophilized Amebocyte Lysate (LAL) eyenziwe yi-Bioendo yenziwe nge-amebocyte lysate ethathwe kwigazi loononkala.
Ikhathalogu No. | Inkcazo | Imixholo yeKit | Uvakalelo EU/ml |
EC80545 | I-Bioendo™ EC Endotoxin Test Kit (Inqaku lokugqibela loVavanyo lweChromogenic, Diazo Coupling), 80 Iimvavanyo / Kit | I-5 ye-Lyophilized Amebocyte Lysate, i-1.7ml / i-vial; 4Amanzi eBET, 50ml/vial; 5 CSE; I-5 Chromogenic Substrate, 1.7ml/vial; 5 Diazo Reagent 1, 10ml/vial; 5 Diazo Reagent 2, 10ml / vial; 5 Diazo Reagent 3, 10ml/vial; | 0.1 - 1 EU / ml |
EC80545S | 0.01 - 0.1 EU / ml; 0.1 - 1 EU / ml |
Uvakalelo lwe-Lyophilized Amebocyte Lysate kunye namandla okuLawula i-Endotoxin esemgangathweni ivavanywa ngokuchasene ne-USP Reference Standard Endotoxin.Iikiti ze-reagent ye-Lyophilized Amebocyte Lysate ziza nomyalelo wemveliso, iSatifikethi sokuHlalutya.
Ngaba i-Endotoxin test kit idinga umfundi ophucukileyo we-microplate?
I-Bioendo EC80545 kunye ne-EC80545S, inokufunda nge-spectrophotometer eqhelekileyo.