IGel Clot Lyophilized Amebocyte Lysate Multi-test Vial G17

I-Gel Clot I-Lyophilized Amebocyte Lysate (LAL) iVial yovavanyo oluninzi, uthotho lwe-G17

1. Ulwazi lweMveliso

I-Gel Clot I-Amebocyte ye-Lyophilized ye-Amebocyte ye-Lyosate ye-Multi-test Vial yi-reagent ye-Lyophilized Amebocyte Lysate ekhetha ubuchule behlwili yejeli ukubona i-endotoxin okanye i-pyrogen.Njengendlela ebanzi, uvavanyo lwe-gel-clot lwe-endotoxin lulula kwaye alufuni isixhobo esikhethekileyo nesibizayo.I-Bioendo ibonelela ngeClot yeGeluvavanyo lwe-endotoxinikhithi kwi-1.7ml kwivial nganye.

2. IParamitha yeMveliso

Uluhlu lobuthathaka: 0.03EU/ml, 0.06EU/ml, 0.125EU/ml, 0.25EU/ml, 0.5 EU/ml

3. Isicelo seMveliso

I-endotoxin yemveliso yokugqibela (pyrogen) isiqinisekiso, amanzi okuhlambauvavanyo lwe-endotoxin, Impahla engekasilwauvavanyo lwe-endotoxinokanye ukubeka iliso kwinqanaba le-endotoxin ngexesha lenkqubo yokuvelisa iinkampani zamachiza okanye abavelisi bezixhobo zonyango.

Qaphela: I-Lyophilized Amebocyte Lysate (i-LAL reagent) eyenziwa yi-Bioendo yenziwe nge-lysate ye-amebocytes (iiseli zegazi ezimhlophe) ukusuka ku-crab we-horseshoe.

Indlela yehlwili yeGelUvavanyo lwe-LAL, i-reagent ehlaziyiweyo ye-lysate ifumana ubuncinane iimvavanyo ezili-16 ngevial ​​nganye:

Imeko yemveliso:

I-Lyophilized Amebocyte Lysate reagent sensitivity kunye ne-Control Standard Endotoxin potency ivavanywa ngokuchasene ne-USP Reference Standard Endotoxin.Iikiti ze-reagent ye-Lyophilized Amebocyte ziza nomyalelo wemveliso, iSatifikethi sokuHlalutya.

Enye yezinto eziphambili ze-Bioendo Lysate reagent ye-G17 yochungechunge oluluqilima lokumelana nokuphazamiseka.Oku kubaluleke ngakumbi xa usebenza ngeesayizi ezinkulu zeesampulu, njengoko kukho amathuba aphezulu ophazamiseko olunokubakho oluvela kwezinye izinto ezikhoyo kwisampulu.Iukufunyanwa kwe-endotoxininkqubo idinga iqondo eliphezulu lovakalelo kunye neenkcukacha, kwaye i-reagent ye-Bioendo Lysate iye yaqulunqwa ngokukodwa ukunciphisa impembelelo yezinto eziphazamisayo.Oku kuqinisekisa ukuba inkqubo yokubona i-endotoxin ichanekile kwaye ithembekile, naxa usebenza ngeesampulu ezinkulu.

Ukongeza kokuchasana nokuphazamiseka, i-Gel Clot Method Lyophilized Amebocyte Lysate Multi-test Vial G17 nayo yenzelwe ukukhululeka kokusetyenziswa.Ifomati ye-lyophilized ye-reagent ivumela ukuzinza kwexesha elide kunye nokugcinwa okulula, ngelixa i-multi-test vial format yenza uvavanyo olufanelekileyo lweesampuli ezininzi.Oku kwenza i-reagent ye-Bioendo Lysate ibe yinto esebenzayo kunye nexabiso eliphantsi lokuchongwa kwe-endotoxin kwizicelo ezinkulu, ezifana nokuveliswa kwamayeza kunye nokuveliswa kwezixhobo zonyango.Ngokubanzi, iUvavanyo lweGel Clot LAL, usebenzisa i-reagent ye-Bioendo Lysate, sisixhobo esibalulekileyo sokuqinisekisa ukhuseleko kunye nomgangatho weemveliso ngokufumanisa ukungcola kwe-endotoxin kwiimali ezinkulu.

Kutheni i-microbiologists ezininzi ukukhetha ikhithi ye-gel clot assay G17 series?

1. Uchungechunge lwe-LAL reagent ye-G17 yeyona ndlela yehlabathi jikelele yokubonisa ubukho be-endotoxins ngokusekelwe ekubeni i-gel yenziwe kwi-tube yesampuli xa isebenzauvavanyo lwebhaktiriya endotoxin.

2. Ezininziuvavanyo lwe-limulus lysateukubhaqa i-endotoxin kumachiza e-parenteral, xa ubhaqa iisampuli zobungakanani obuphakathi.

3. Inkqubo yovavanyo lwe-LAL yokufukamela, isixhobo esifanelekileyo kukuhlamba ngamanzi okanye i-incubator yokushisa eyomileyo.

4. Umgangatho ophezulu wokuphela kwe-endotoxin ityhubhu yamahhala (<0.005EU / ml) kunye nomgangatho ophezulu we-pyrogen tip yamahhala (<0.005EU / ml) njengento yokusetyenziswa okuqinisekisiweyo ukuqinisekisa umphumo ochanekileyo.

Iimveliso ezinxulumeneyo kuvavanyo lovavanyo lwe-endotoxin:

• Amanzi oVavanyo lwe-Endotoxins ye-Bacterial (BET), Cebisa i-TRW50 okanye i-TRW100
• I-Endotoxin yasimahla ityhubhu yeglasi (ityhubhu yokuxutywa), Ncoma i-T1310018 kunye ne-T107540
• Iingcebiso zamahhala zePyrogen, Ncoma i-PT25096 okanye i-PT100096
• Pipettor, Ncoma iPSB0220
• Uvavanyo lweTube Rack
• Isixhobo sokufukamela (iBhafu yamanzi okanye i-Incubator yobushushu obomileyo), cebisa i-Dry Heat Incubator TAL-M2
• Umxube weVortex, Ncoma iVXH.
• Ukulawula i-Endotoxin esemgangathweni, i-CSE10V.

I-BioendoUvavanyo lwe-LAL lwepyrogens or Uvavanyo lwe-LAL endotoxin, kwayeUvavanyo lwe-TAL, zonke iinkcazo zezovavanyo lwe-endotoxin.

Isiseko kwi-Bioendo processing art, iikhithi zi lyophilized amebocyte lysate (LAL).

Limulus amebocyte lysate (LAL) yincindi emanzi kwiiseli zegazi (ii-amoebocytes) isuka eAtlantic horseshoe unonkala I-Limulus polyphemus.I-LAL isabela ngebhaktiriyaendotoxin lipopolysaccharide(LPS), eyiinwebuinxalenye yeiintsholongwane zegram-negative.Le mpendulo isisiseko seUvavanyo lwe-LAL, esetyenziswa ngokubanzi ukufumanisa kunye nobungakanani bebhaktheriyaii-endotoxins.

EAsia, okufanayoTachypleus amebocyte lysate (TAL) uvavanyo olusekwe kuononkala basekhaya boononkalaTachypleus gigas or I-Tachypleus tridentatusngamanye amaxesha isetyenziswa endaweni yoko.Ii-recombinant factor C(rFC) uvavanyo aukutshintshwaye-LAL/TAL esekwe kwimpendulo efanayo.