I-Gel Clot I-Lyophilized Amebocyte Lysate Uvavanyo olulodwa kwi-Vial Umfanekiso obonakalayo

I-Gel Clot Lyophilized Amebocyte Lysate Uvavanyo olulodwa kwiVial

I-Gel Clot I-Lyophilized Amebocyte Lysate Uvavanyo olulodwa kwi-Vial iqulethe i-0.2ml endotoxin-specific Amebocyte Lysate equka i-beta-glucan inhibitor ekuqulunqweni kwaye ayiyi kusabela kwi-beta-glucan.Ngaphaya koko, isampulu inokongezwa kwi-vial ngokuthe ngqo.Oku kuthetha ukuba awudingi ukuphinda uhlanganise i-Amebocyte Lysate ekuqaleni, kwaye unokugqiba ukuba zingaphi iibhotile ezisetyenziselwa ixesha ngalinye ukunqanda inkcitho.

Ukubuza

Iinkcukacha zeMveliso

IGqeba leGel LyophilizedAmebocyte LysateUvavanyo olulodwa kwiVial

1. Ulwazi lweMveliso

IGqeba leGel LyophilizedAmebocyte LysateUvavanyo olulodwakwiVial Yiba ne-endotoxin-specific Amebocyte Lysate equka i-beta-glucan inhibitor ekuqulunqweni kwaye ayiyi kusabela kwi-beta-glucan.Kuvavanyo lwethu olunye kwiVial, unokongeza isampuli kwi-vial ngokuthe ngqo.Oku kuthetha ukuba awudingi ukuphinda uhlanganise i-Amebocyte Lysate ekuqaleni, kwaye uya kuthatha isigqibo sokuba zingaphi iibhotile ezisetyenziselwa ixesha ngalinye ukuphepha inkunkuma.I-Endotoxin yasimahla ityhubhu yokuhlanjululwa kwe-CSE iyimfuneko.Ukusebenza kweukufunyanwa kwe-endotoxinngokukhetha i-Bioendo Gel Clot I-Lyophilized Amebocyte Lysate Uvavanyo olulodwa kwi-Vial ihambelana ne-USP, i-EP.

2. IParamitha yeMveliso

I-Gel clot assay yokuvavanya iglasi enye yeglasi

Uvakalelo: 0.03EU/ml, 0.06EU/ml, 0.125EU/ml, 0.25 EU/ml

3.Uphawu lweMveliso kunye neSicelo

Inyathelo elinyeukufunyanwa kwe-endotoxin,

ngaphandle kwexabiso eliphezuluuvavanyo lwe-endotoxinizixhobo,

Ifanelekileisiphelo-mvelisouvavanyo lwe-endotoxinngaphambi kokuba imveliso ikhutshwe,

Uvakalelo lwemveliso lubekwe emgangathweni ngokwemigaqo ye-US Pharmacopoeia kunye ne-China Pharmacopoeia criterion.

Phawula:

I-reagent ye-Lyophilized Amebocyte Lysate (LAL) eyenziwa yi-Bioendo yenziwe nge-lysate ye-amebocytes (iiseli zegazi ezimhlophe) ukusuka ku-crab we-horseshoe.

Inombolo yekhathalogu	Uvakalelo (EU/ml) (IU/ml)	Iimvavanyo / Vial	Iingqayi / Pack
G020015	0.015	1	50
G020030	0.03	1	50
G020060	0.06	1	50
G020125	0.125	1	50
G020250	0.25	1	50
G020500	0.5	1	50

Imeko yeMveliso

Uvakalelo lwe-Lyophilized Amebocyte Lysate kunye namandla okuLawula i-Endotoxin esemgangathweni ivavanywa ngokuchasene ne-USP Reference Standard Endotoxin.Iikiti ze-reagent ye-Lyophilized Amebocyte Lysate ziza nomyalelo wemveliso, iSatifikethi sokuHlalutya.

Kutheni eyona khithi yokuvavanya ihlwili yejeli G02:

1. Eyona nto inoqoqosho kwaye isetyenziswa ngokuqhelekileyo i-reagent yokuvavanya ukufumanisa i-endotoxin.

2. Uvavanyo olunye kwi-vial ukwenzela ukunyathela okunye kunciphisa umngcipheko wosulelo.

3. I-Gel clot assay yeglasi yovavanyo enye yeglasi akukho mfuneko yomfundi we-microplate ephucukileyo.

4. Ukugcina ityhubhu yasimahla ye-endotoxin xa usebenzisa uchungechunge lwe-G02 ukuvavanya i-endotoxins kwinkqubo yovavanyo lovavanyo lwe-endotoxin.

Iimveliso ezinxulumeneyo kuvavanyo lovavanyo lwe-endotoxin:

Amanzi oVavanyo lwe-Endotoxins ye-Bacterial (BET), Cebisa i-TRW50 okanye i-TRW100

I-Endotoxin ityhubhu yeglasi yasimahla (i-tube dilution), Ncoma i-T1310018

Iingcebiso zamahhala zePyrogen, Ncoma i-PT25096 okanye i-PT100096

Pipettor, Ncoma iPSB0220

Uvavanyo lweRakhi yeTubhu,

Isixhobo sokufukamela (iBhafu yamanzi okanye i-Incubator yobushushu obomileyo), cebisa i-Dry Heat Incubator TAL-M2

Umxube weVortex, Ncoma iVXH.

Ukulawula i-Endotoxin esemgangathweni, i-CSE10V.

Ngaphambili: I-Gel Clot Lyophilized Amebocyte Lysate Uvavanyo olunye kwi-Ampoule GS44

Okulandelayo: IKit yoVavanyo ye-Bioendo GC Endotoxin (i-Gel Clot Assay)

Shiya iMiyalezo Yakho

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