Ubuchule beChromogenic buphakathi kobuchule obuthathu obukwaqulethe ubuchule bejeli-clot kunye nobuchule be-turbidimetric bokubona okanye ukulinganisa ubungakanani be-endotoxins kwi-Gram-negative bacteria ngokusebenzisa i-amoebocyte lysate etsalwe kwigazi elizuba loononkala (i-Limulus polyphemus okanye i-Tachypleus tridentatus).Inokuthi ihlelwe njenge-endpoint-chromogenic assay okanye i-kinetic-chromogenic assay esekelwe kumgaqo othile wokuvavanya osetyenzisiweyo.
Umgaqo wokusabela kukuba: i-amebocyte lysate iqulethe i-cascade ye-serine protease enzymes (i-proenzymes) enokuthi isebenze yi-endotoxins yebhaktiriya.I-Endotoxins ivuselela i-proenzymes ukuvelisa i-enzymes esebenzayo (ebizwa ngokuba yi-coagulase), le yokugqibela ibangela ukwahlukana kwe-substrate engenambala, ikhupha i-pNA enemibala ephuzi.I-pNA ekhutshiweyo inokulinganiswa nge-photometrically kwi-405nm.Kwaye ukufunxa kunxulunyaniswa ngokufanelekileyo kunye noxinaniso lwe-endotoxin, emva koko ugxininiso lwe-endotoxin lunokubalwa ngokufanelekileyo.
Ixesha lokuposa: Sep-29-2019
