IsikhokeloyeBioendo End-point Chromogenic LAL Test Assay Kits:
I-reagent ye-TAL, oko kukuthi i-lyophilized amebocyte lysate etsalwa kwigazi eliblowu likanonkala wehashe (i-Limulus polyphemus okanye i-Tachypleus tridentatus), isoloko isetyenziselwa ukwenza uvavanyo lwe-bacterial endotoxins.
Kwi-Bioendo, senza iikiti zokwenza i-end-point chromogenic TAL assay / LAL assay, ebandakanya zonke ii-reagents eziyimfuneko kwi-BET ngotshintsho kuphuhliso lombala, kwaye uvumele umthengi ukuba afumane iziphumo ezichanekileyo ngexesha elifutshane.
Imigaqo yokufumanisa i-chromogenic ye-end-point kunye ne-TAL reagent yile: ukusabela kwe-enzymatic kwenzeka xa i-endotoxins yebhaktheriya ivuselela i-C, kwaye i-coagulase yenziwe ngokufanelekileyo.Emva koko i-coagulase yahlula i-substrate engenambala ukuze ikhuphe imveliso enombala omthubi ipNA.I-pNA ekhutshiweyo inokulinganiswa ngokufotayo kwi-405nm.Kuba ukufunxa kunxulunyaniswa ngokufanelekileyo kunye noxinaniso lwe-endotoxin, ugxininiso lwe-endotoxin lunokubalwa ngokufanelekileyo.
EC64405, oko kukuthi iKhiti yoVavanyo lwe-BioendoEC Endotoxin (i-End-point Chromogenic Assay), isiphelo se-Chromogenic TAL assay / LAL ikhithi yokuvavanya enokuthi isetyenziswe ukwenza ukufumanisa ubuninzi be-bacterial endotoxin ekhoyo kwisampulu.I-EC64405 ikhithi eveliswe yi-Bioendo ivumela iimvavanyo ze-64, kunye nobukhulu bezinga ukusuka kwi-0.1 EU / ml ukuya kwi-1 EU / ml.I-Bioendo EC64405 iqulethe zonke ii-reagents ezifunekayo kwi-end-point assay chromogenic.Kuba sikwanazo ne-endotoxin-free okanye i-pyrogen-free accessories, sinokupakisha ikhithi ngokusekelwe kwinto oyifunayo.
I-Incubating Microplate Reader iyafuneka kwi-end-point assay chromogenic.Umfundi wethu we-microplate incubating ELx808IULALXH (imodeli entsha ephantsi kokulungiswa kwaye iya kuqaliswa kungekudala) ivumela iisampuli ezahlukeneyo ukuba zibonwe kwi-96-well microplate ngexesha elifanayo kwaye iya kuhlalutya ukufunyanwa kwe-endotoxin ngokuzenzekelayo nangokuchanekileyo.
Isiphelo sovavanyo lovavanyo lwe-chromogenic ye-LAL kunye nothotho lwekiti ezimbini, enye isebenzisa umfundi we-microplate ukufunda isiphumo sobungakanani nge-pyrogen-free microplate, enye isebenzisa i-spectrophotometer eqhelekileyo ukufunda isiphumo sobungakanani ngeetyhubhu zeglasi ezingenayo i-endotoxin.
I-Lyophilized Amebocyte Lysate / LAL Reagent eyenziwe yi-Bioendo yenziwe nge-amebocyte lysate ethathwe kwigazi loononkala.
| Inombolo yekhathalogu. | Inkcazo | Imixholo yeKit | Uvakalelo (EU/ml) |
| EC64405 |
IKit yoVavanyo lwe-Bioendo EC Endotoxin (i-End-point Chromogenic Assay), Iimvavanyo ezingama-64/Ikhithi
| I-2 ye-Lyophilized Amebocyte Lysate, i-1.7ml / i-vial; 2 Amanzi eBET, 50ml/vial; 2 CSE; I-4 Chromogenic Substrate, 1.7ml / vial; | 0.1 - 1 EU / ml |
| EC64405S | 0.01 - 0.1 EU / ml; 0.1 - 1 EU / m |
I-Lyophilized Amebocyte Lysate / LAL Reagent eyenziwe yi-Bioendo yenziwe nge-amebocyte lysate ethathwe kwigazi loononkala.
| Inombolo yekhathalogu. | Inkcazo | Imixholo yeKit | Uvakalelo EU/ml |
| EC80545 | I-Bioendo EC Endotoxin Test Kit (Inqaku lokugqibela loVavanyo lweChromogenic, Diazo Coupling), 80 Iimvavanyo / Kit | I-5 ye-Lyophilized Amebocyte Lysate, i-1.7ml / i-vial; 4 Amanzi eBET, 50ml/vial; 5 CSE; I-5 Chromogenic Substrate, 1.7ml/vial; 5 Diazo Reagent 1, 10ml/vial; 5 Diazo Reagent 2, 10ml / vial; 5 Diazo Reagent 3, 10ml/vial; | 0.1 - 1 EU / ml |
| EC80545S | 0.01 - 0.1 EU / ml; 0.1 - 1 EU / ml |
Ixesha lokuposa: Sep-15-2019
