Iityhubhu zeglasi kunye ne-depyrogenation processing ziyimfuneko kwi-endotoxin test assay ukuqinisekisa ukuchaneka kunye nokuthembeka kweziphumo zovavanyo.I-Endotoxins zizinto zemolekyuli ezizinzile ngobushushu zodonga lwangaphandle lweebhaktheriya ezinegram-negative, kwaye zinokubangela ukugula kakhulu kunye nokufa ebantwini ukuba zikhona kwiimveliso zonyango okanye izixhobo.
Ukubona i-endotoxins, i-assay isebenzisa i-reagents equkethe i-Limulus Amebocyte Lysate (LAL) okanye ebizwa ngokuba yi-Lyophilized amebocyte lysate, isicatshulwa esivela kwiiseli zegazi ze-crab yehashe ene-clotting mechanism eyenziwa yi-endotoxins.Nangona kunjalo, iityhubhu zeglasi ezingakhange zisuswe i-depyrogenated zinokuphazamisana ne-assat yovavanyo lwe-LAL ngokuvula indlela yayo yokujika kunye nokuvelisa iziphumo ezingezizo ezingezizo.Ke ngoko, iityhubhu zeglasi ezisetyenziswa kuvavanyo lovavanyo lwe-endotoxin kufuneka zikhunjulwe ukuze zisuse naziphi na i-endotoxins ezinokuthi zibekhona kunye nokuthintela ukusebenza kwe-reagent ye-LAL.Oku kuqinisekisa ukuba iziphumo zovavanyo lwe-endotoxin zichanekile kwaye zithembekile kwaye izigulane azibonakali kumanqanaba ayingozi e-endotoxins.kunye nokuqinisekisa ukhuseleko lwamachiza e-parenteral kwi-pharmaceuticals, iiprotheyini, inkcubeko yeeseli, i-DNA njalo njalo.
Imfuneko yokusetyenziswa kweetyhubhu zeglasi ezingenayo i-endotoxin kumsebenzi wokufumanisa i-endotoxin:
Iityhubhu zeglasi ezingenayo i-endotoxinziyinxalenye ebalulekileyo yalo naluphi na uvavanyo lwe-endotoxin.Ezi tyhubhu zeglasi zenzelwe ukunciphisa umngcipheko wokungcoliseka kwe-endotoxin ngexesha lenkqubo yovavanyo, ukuqinisekisa iziphumo ezichanekileyo nezithembekileyo.
Enye yezinto eziphambili zeetyhubhu zeglasi ezingenayo i-endotoxin kukwakheka kweekhemikhali.Ezi tyhubhu zenziwe ngeglasi ye-borosilicate ekumgangatho ophezulu, eyaziwa ngokuxhathisa okukhethekileyo kwi-corrosion yeekhemikhali.Oku kubenza balungele ukusetyenziswa kuvavanyo lwe-endotoxin, njengoko banokumelana nokuvezwa kuluhlu olubanzi lweekhompawundi zovavanyo ngaphandle kokuthoba okanye ukungcolisa isampuli.
Olunye uphawu olubalulekileyo lwe-endotoxin-free glass tubes kucoceko lwazo.Le mibhobho icocwa ngononophelo kwaye ivalwe iintsholongwane phambi kokuba isetyenziswe ukususa nayiphi na imithombo enokubakho yongcoliseko.Zikwavavanyelwa ngokungqongqo ukungcoliseka kwe-endotoxin, ukuqinisekisa ukuba azinaso naziphi na izixa zale nto iyingozi.
Ukongeza, ii-endotoxin-free glass tubes ziyilelwe ukuba zibe lula ukuzisebenzisa.Ngokuqhelekileyo zifumaneka kuluhlu lweesayizi kunye nolungelelwaniso ukulungiselela iisampulu ezahlukeneyo kunye neendlela zokuvavanya zombini uvavanyo lwe-endotoxin yomgangatho kunye novavanyo lobungakanani be-endotoxin.Zikwahambelana neentlobo ezahlukeneyo zesampulu zamalungiselelo kunye nezixhobo zovavanyo, zizenza zibe lukhetho oluguquguqukayo nolufanelekileyo kwiilabhoratri zovavanyo lwe-endotoxin.
Ngokubanzi, iityhubhu zeglasi ezingenazi-endotoxin zidlala indima ebalulekileyo ekuchanekeni nasekuthembekeni kovavanyo lwe-endotoxin.Ukwakhiwa kwabo okukumgangatho ophezulu, ukucoceka, kunye nokusebenziseka ngokulula kubenza babe yinxalenye ebalulekileyo yalo naluphi na uvavanyo oluyimpumelelo lwe-endotoxin.
Iityhubhu zeglasi zeBioendo Endotoxin ezinobungakanani10*75mm, 12*75mm, 13*100mm kunye 16*100mmkwiinkqubo zokuhlanjululwa kunye neenkqubo zokusabela.
Iityhubhu zeglasi ezingenayo i-Endotoxin zidibana nomgangatho ophezulu we-endotoxins ngaphantsi kwe-0.005EU/ml.
Iityhubhu zeglasi ezingenazo i-Endotoxin kufuneka zisetyenziswe kwi-gel clot endotoxin test assay ukuthintela iziphumo ezingezizo.
I-Endotoxins ngamalungu odonga lweeseli zebhaktiriya anokuthi angcolise izixhobo zelabhoratri, kuquka iityhubhu zeglasi.
Uvavanyo lwe-gel clot endotoxin uvavanyo lusetyenziselwa ukubona ubukho be-endotoxins kwisampulu.Kolu vavanyo, ihlwili lenziwa phambi kwee-endotoxins.Olu kwakheka kwehlwili luze luthelekiswe nolawulo lokumisela ukuxinana kwe-endotoxin.
Ukusebenzisa iityhubhu zeglasi ezingenayo i-endotoxin kunceda ukuqinisekisa ukuba ukubonwa kwe-endotoxin kuchanekile.Oku kungenxa yokuba i-endotoxins inokubambelela kumphezulu weetyhubhu zeglasi kwaye iphazamise iziphumo zovavanyo.
Ukuqinisekisa ukuba iityhubhu zeglasi ezisetyenziswe kuvavanyo lwe-gel clot endotoxin test assay-endotoxin-free, kufuneka zihlanjwe ngesicoci kwaye zihlanjwe kakuhle ngamanzi angenayo i-endotoxin.Ukongeza, kufuneka zicocwe kusetyenziswa i-autoclaving okanye inzalo eyomileyo yobushushu.
Ukuqukumbela, ukusebenzisa iityhubhu zeglasi ezingenayo i-endotoxin kwi-gel clot endotoxin test assay kubalulekile ukuqinisekisa ukufunyanwa ngokuchanekileyo kwe-endotoxins.Le mibhobho kufuneka icocwe ngokucokisekileyo kwaye ivalwe iintsholongwane ukuphelisa naluphi na ungcoliseko olunokubakho.
Ixesha lokuposa: Jun-02-2023