Amanzi angenayo i-Endotoxin adlala indima ebalulekileyo ekuchanekeni nasekuthembekeni komsebenzi wokuvavanya i-endotoxin.I-Endotoxins, ekwaziwa ngokuba yi-lipopolysaccharides (LPS), zizinto eziyityhefu ezikhoyo kwiindonga zeeseli ze-Gram-negative bacteria.Ezi zingcolisi zinokubangela ingozi enkulu ebantwini nakwizilwanyana ukuba azisuswanga kwiimveliso zonyango ezifana nogonyo, amachiza, kunye nezixhobo zonyango.
Ukubona kunye nokulinganisa amanqanaba e-endotoxin ngokuchanekileyo, uvavanyo lwe-endotoxin luxhomekeke kuvavanyo olubuthathaka olufuna ukusetyenziswa kwamanzi angenayo i-endotoxin.Olu hlobo lwamanzi luphathwa ukuze lususe zonke iimpawu ze-endotoxins, ukuqinisekisa ukuba naziphi na iziphumo ezilungileyo eziveliswa yi-assay zibangelwa kuphela ubukho be-endotoxins kwisampuli evavanywayo, kwaye kungekhona umphumo wokungcola emanzini.
Ukusebenzisa amanzi angenayo i-endotoxin kwakhona kunceda ukunciphisa iziphumo ezilungileyo zobuxoki, ezinokuthi zenzeke xa kukho umkhondo we-endotoxins emanzini asetyenziswe kuvavanyo.Oku kunokukhokelela kwiziphumo ezingachanekanga, ezinokubangela ukulibaziseka ekukhutshweni kwemveliso kunye nemiba yokulawula.
Isishwankathelo, amanzi angenayo i-endotoxin yinxalenye ebalulekileyo yovavanyo lovavanyo lwe-endotoxin, eqinisekisa ukuchaneka kunye nokuthembeka kolu vavanyo lubalulekileyo.Ngokunciphisa umngcipheko wobuxoki kunye nokuqinisekisa ukuba iziphumo ezilungileyo ziveliswa kuphela xa kukho ungcoliseko lokwenyani lwe-endotoxin, amanzi angenayo i-endotoxin adlala indima ebalulekileyo ekuqinisekiseni ukuba iimveliso zonyango zikhuselekile kwaye ziyasebenza kwizigulana.
Amanzi ovavanyo lwebhaktheriya endotoxin
Umahluko phakathi kwamanzi ovavanyo lwe-endotoxin yebhaktiriya kunye namanzi angenazintsholongwane enaliti: i-pH, i-endotoxin yebhaktiriya kunye nezinto eziphazamisayo.
Amanzi ovavanyo lwebhaktheriya endotoxin
Umahluko phakathi kwamanzi ovavanyo lwe-endotoxin yebhaktiriya kunye namanzi angenazintsholongwane enaliti: i-pH, i-endotoxin yebhaktiriya kunye nezinto eziphazamisayo.
1. pH
Eyona pH ifanelekileyo yokusabela phakathiLAL reagentkunye ne-endotoxin yi-6.5-8.0.Ngoko ke, kuvavanyo lwe-LAL, eUnited States, i-Pharmacopoeia yaseJapan kunye ne-2015 edition ye-Chinese Pharmacopoeia ichaza ukuba ixabiso le-pH lemveliso yovavanyo kufuneka lihlengahlengiswe kwi-6.0-8.0.Ixabiso le-pH lamanzi lokuvavanya i-endotoxin yebhakteria ilawulwa ngokubanzi kwi-5.0-7.0;ixabiso le-pH lamanzi angenazintsholongwane okutofa kufuneka lilawulwe kwi-5.0-7.0.Kuba uninzi lwamayeza aneasidi ebuthathaka, ixabiso le-pH lamanzi kuvavanyo lwe-endotoxin yebhaktiriya lilungele uvavanyo lovavanyo lwe-endotoxin okanye uvavanyo lwe-Lyophilized amebocyte lysate.
2. I-Bacterial Endotoxin
Ubungakanani be-endotoxin emanzini kuvavanyo lwe-endotoxin yebhakteria kufuneka ibe ubuncinane ngaphantsi kwe-0.015EU nge-1ml, kwaye inani le-endotoxin emanzini kuvavanyo lwe-endotoxin yebhakteria kwiindlela zobuninzi kufuneka ibe ngaphantsi kwe-0.005EU nge-1ml;Amanzi angenazintsholongwane okutofwa kufuneka aqulathe ngaphantsi kwe-0.25 EU ye-endotoxin nge-1ml nganye.
I-endotoxin emanzini yovavanyo lwe-endotoxin yebhaktiriya kufuneka ibe sezantsi ngokwaneleyo ukuba ingachaphazeli iziphumo zovavanyo.Ukuba kusetyenziswe amanzi acocekileyo okutofwa endaweni yamanzi ovavanyo lovavanyo lwe-Endotoxin, ngenxa yesiqulatho esiphezulu se-endotoxin kumanzi angenanto yokutofwa, amanzi anyumba enaliti kunye ne-Superposition ye-endotoxin kwisampulu evavanyiweyo inokuvelisa iziphumo ezingezizo, ezibangela ilahleko yezoqoqosho ngokuthe ngqo. kwishishini.Ngenxa yomahluko kumxholo we-endotoxin, akunakwenzeka ukusebenzisa amanzi angenazintsholongwane kwinaliti endaweni yokuhlola amanzi ovavanyo lovavanyo lwe-endotoxin okanye i-Lyophilized amebocyte lysate test assay.
3. Izinto eziphazamisayo
Amanzi ovavanyo lwe-endotoxin yebhaktiriya akufuneki aphazamise i-reagent ye-LAL, ukulawula umgangatho we-endotoxin kunye novavanyo lwe-LAL;akukho mfuneko yamanzi angenazintsholongwane okutofwa.Amanzi anyumba enaliti afuna ukhuseleko kunye nozinzo, kodwa ngaba amanzi anyumba enaliti ayawuchaphazela umsebenzi kunye nokuzinza kwe-endotoxin yomgangatho wolawulo lwebhaktiriya?Ngaba Amanzi aNtsholongwane okutofwa ayaluphucula okanye alunqande uvavanyo lwe-endotoxin?Bambalwa abantu abenze uphando lwexesha elide malunga noku.Kuye kwaqinisekiswa ngophando ukuba amanzi acwengileyo enaliti anempembelelo enamandla yokuthintela kuvavanyo lwe-LAL.Ukuba kusetyenziswe amanzi acocekileyo enaliti endaweni yamanzi ovavanyo kuvavanyo lwe-LAL, izinto ezingezizo ezinokuthi zenzeke, ezikhokelela ekuphosweni kwe-endotoxin, ebeka emngciphekweni ukhuseleko lwamayeza ngokuthe ngqo.Ngenxa yobukho bemiba yophazamiseko lwamanzi angenazintsholongwane okutofwa, akwenzeki ukuba kusetyenziswe amanzi angenazintsholongwane ekutofweni endaweni yokuhlola amanzi kuvavanyo lwe-LAL.
Ukuba ukuchaneka kwamanzi okuhlamba, indlela yokuhlamba kunye namanzi okuvavanya kunokuqinisekiswa, ukuba kunokwenzeka ukuba ulawulo olulungileyo kuvavanyo lweLimulus alukwazi ukusekwa ngokusisiseko alukho, ngaphandle kokuba umgangatho osetyenzisiweyo awufani.Ukuze siqinisekise ukuchaneka kweziphumo zovavanyo, kufuneka:
a.Uqhelene nemigangatho kunye nemigaqo yoshishino;
b.Sebenzisa iimveliso ezifanelekileyo kunye neemveliso eziqhelekileyo;
c.Sebenza ngokungqinelana nemigaqo yokusebenza.
Ixesha lokuposa: Jul-26-2023
