Uvavanyo lwe-endotoxin yebhaktiriya (BET) lwenziwa kwiilabhoratri zanamhlanje phantsi kweemeko ezilawulwayo njengeyona nto ibalulekileyo yokuphepha ukuphazamiseka.
Ifanelekileubuchule be-aseptickubalulekile xa ulungiselela kwaye udibanisa imigangatho kunye neesampuli zokuphatha.Ukunxibaukuziqhelanisa ngaphandle kwezixhobo eziqhelekileyo zokuzikhusela kwilabhoratri (PPEIimfuno aziyongxaki ngaphandle kokuba imveliso ephantsi kovavanyo ifuna iingqwalasela ezikhethekileyo zokhuseleko lomhlalutyi ngenxa yetyhefu okanye ukosuleleka.Iiglavukufuneka ingabi yi-TALC, njengoko i-TALC inokuqulatha amanqanaba abalulekileyo e-endotoxins.Iipleyiti zokufunda, iibhafu zamanzi, kunye neebhloko zobushushu ezomileyoesetyenziselwa ufukamo lwesampulu kufuneka ibe kwibhentshi yaselabhoratri kude nemigudu yokufudumeza, yokungena komoya, kunye neyomoya (HVAC), ukungcangcazela okubalulekileyo, kunye nokugcwala kwelebhu ezinokuchaphazela iziphumo zovavanyo.Amaxesha kunye neemeko zokubamba iisampulukufuneka kumiselwe kwaye emva koko kubhalwe phantsi, ukuba kukho imfuneko, ukuqinisekisa ukuba iziphumo zovavanyo ezichanekileyo zinokwenziwa ngexesha elifanelekileyo.
Umzekelo, ukuba ilabhoratri ifumana Amanzi okuTofwa (WFI) okanye isampulu esele isenziwa, kufuneka ifakwe efrijini okanye ihlale kubushushu begumbi, kwaye ixesha elingakanani?Phambi kovavanyo, kucetyiswa ukuba isikhongozeli sesampulu sokuqala (izikhongozeli) sixutywe ngokwaneleyo phambi kokuba kususwe uvavanyo lwe-aliquot(s) ukwenzela nokuba luvavanyo oluthe ngqo okanye luxutywe.
Uvavanyo lwe-Bioendo Bacterial Endotoxin, iimvavanyo zibandakanyaindlela yehlwili yejeliendotoxin uvavanyo assay kunyeUvavanyo lovavanyo lwe-endotoxin yobungakanani, i-gel clot indlela yokuvavanya i-endotoxin yokuhlola i-endotoxin esemgangathweni, olu vavanyo lufuna izinto ezisetyenziswayo yi-depyrogenation processing, njenge-endotoxin yeetyhubhu zokusabela ezikhululekile, iityhubhu zokuxutywa kunye neengcebiso zamahhala zepyrogen;Ukufunyanwa kwe-endotoxin yobungakanani kunovavanyo lwe-kinetic chromogenic endotoxin, uvavanyo lwe-kinetic turbidimetric endotoxin, olu vavanyo lufuna izinto ezityiwayo kufuneka zihlangabezane nenqanaba eliphezulu leendotoxins ngaphantsi kwe.0.005EU/ml( 0.001EU / ml ), ezifana ne-endotoxin yamahhala, iingcebiso zamahhala ze-pyrogen, kunye ne-pyrogen yamahhala ye-microplates, kunye ne-pyrogen free revoir.Ngendlela, ukuba iisampulu zonyango, isikhongozelo kufuneka ibe yibhotile yesampulu yasimahla ye-endotoxin.
Kuvavanyo lwe-endotoxin, ukuphazamiseka kunokuvela kwimithombo eyahlukeneyo, enje ngesampulu yesampulu ye-matrix, ii-reagents zovavanyo, okanye izixhobo.
Ukunqanda uphazamiseko lomfuniselo, la manyathelo alandelayo anokuthathwa:
1. Ukulungiselela iSampuli: Ukulungiswa okufanelekileyo kwesampuli kubalulekile kuvavanyo oluchanekileyo lwe-endotoxin.
Isampulu yematrix kufuneka ivavanywe ngokucokisekileyo kwaye iphuculwe ukuqinisekisa ukuhambelana novavanyo lwe-endotoxin.
Ngokukodwa, izinto eziphazamisayo ezifana ne-lipids kunye neeprotheni kufuneka zisuswe okanye zincitshiswe kusetyenziswa iindlela ezifanelekileyo ezifana nokuhluza okanye i-centrifugation.
2. Ulawulo oluhle kunye nolungalunganga: Kubalulekile ukubandakanya ulawulo olulungileyo kunye olubi kwi-assay ukubeka iliso ekuphazamiseni.
Ulawulo oluhle luqinisekisa ukusebenza kovavanyo, ngelixa ulawulo olubi lubona naluphi na ungcoliseko okanye ukuphazamiseka kumacandelo ovavanyo.
3. Ukulawulwa komgangatho: Ulawulo lomgangatho kufuneka lwenziwe kuzo zonke ii-reagents, izixhobo, kunye namanzi asetyenziswa kuvavanyo.
Oku kuqinisekisa ukuba ii-reagents zikhululekile kungcoliseko lwe-endotoxin kwaye zisebenza ngokuchanekileyo.
4. Ukumiswa komgangatho: Uvavanyo kufuneka lubekwe emgangathweni ukuze kuqinisekiswe ukuba zonke iziphumo ziyathelekiseka kwaye ziphinde zenziwe kwakhona.
Oku kubandakanya ukusetyenziswa kwegophe elisemgangathweni ukulinganisa uvavanyo kunye nokusetyenziswa kobuchule obusemgangathweni ukulungiselela isampulu, ukufukanyelwa kunye nobhaqo.
5. Ukuqinisekiswa: Uvavanyo kufuneka luqinisekiswe ukuqinisekisa ukuba luchanekile, lubuthathaka, kwaye luthembekile.
Oku kubandakanya ukuvavanya uluhlu lweesampulu, kubandakanywa nezo zaziwa ngokuba ziqulethe i-endotoxin, ukumisela ukuchaneka kunye nokuchaneka kovavanyo.
Ngokulandela la manyathelo, ukuphazamiseka kunokuncitshiswa, kwaye uvavanyo oluchanekileyo lwe-endotoxin lunokufezekiswa.
Ixesha lokuposa: Dec-01-2022
