Uvavanyo loVavanyo lwe-Kinetic Chromogenic Endotoxin (uvavanyo lweChromogenic LAL/TAL)

KCET- Kinetic Chromogenic Endotoxin Test Assay (Chromogenic endotoxin test assay yindlela ebalulekileyo yeesampulu ezinokuphazamiseka okuthile.)
Uvavanyo lwe-kinetic chromogenic endotoxin (KCT okanye i-KCET) luvavanyo yindlela esetyenziselwa ukufumanisa ubukho be-endotoxins kwisampulu.
I-Endotoxins zizinto ezinetyhefu ezifumaneka kwiindonga zeeseli zeentlobo ezithile zebhaktheriya, kuquka iibhaktheriya zegram-negative ezifana ne-Escherichia coli kunye ne-Salmonella.Kuvavanyo lwe-KCET, i-substrate ye-chromogenic yongezwa kwisampulu, ephendula ngayo nayiphi na i-endotoxins ekhoyo ukuvelisa utshintsho lombala.
Izinga lophuhliso lombala libekwe esweni ngokuhamba kwexesha kusetyenziswa i-spectrophotometer, kwaye inani le-endotoxin kwisampulu libalwa ngokusekelwe kulo mlinganiselo.
Uvavanyo lwe-KCT yindlela edumileyo yokufumanisa i-endotoxins kumayeza, izixhobo zonyango, kunye nezinye iimveliso ezidibana nomzimba womntu.Luvavanyo olunovakalelo noluthembekileyo olukwaziyo ukubona nezixa ezincinci kakhulu ze-endotoxin, nto leyo eyenza ibe sisixhobo esibalulekileyo sokuqinisekisa ukhuseleko lwezi mveliso.

 

I-TAL/LAL reagent yi-lyophilized amebocyte lysate ekhutshwa kwigazi elibhlowu le-Limulus polyphemus okanye i-Tachypleus tridentatus.

I-Endotoxins zi-amphiphilic lipopolysaccharides (LPS) ezibekwe kwi-membrane yeseli engaphandle yebhaktiriya yegram-negative.Iimveliso zabazali ezingcoliswe yi-pyrogens ezibandakanya i-LPS zinokukhokelela kuphuhliso lomkhuhlane, ukungeniswa kwempendulo yokuvuvukala, ukothuka, ukusilela kwamalungu kunye nokufa ebantwini.
Ke ngoko, amazwe kwihlabathi liphela aqulunqe imimiselo, efuna ukuba nayiphi na imveliso yechiza ethi ayinyumba kwaye ayinayo i-pyrogenic kufuneka ivavanywe ngaphambi kokukhululwa.Uvavanyo lwe-Gel-clot TAL lwaphuhliswa okokuqala kuvavanyo lwe-bacterial endotoxins (okt BET).
Nangona kunjalo, ezinye iindlela ezihambele phambili ngakumbi zovavanyo lwe-TAL ziye zavela.Kwaye ezi ndlela aziyi kubhaqa kuphela kodwa ziphinde zilinganise ubukho be-endotoxins kwisampulu.Ngaphandle kobuchule behlwili yejeli, ubuchule be-BET bukwaqulathe ubuchule be-turbidimetric kunye nobuchule bechromogenic.I-Bioendo, ezinikele ekubhaqweni kwe-endotoxin, ngumvelisi oqeqeshiweyo wokuphuhlisa i-chromogenic TAL/LAL assay.
I-Bioendo EC Endotoxin Test Kit (I-End-point Chromogenic Assay) ibonelela ngomlinganiselo okhawulezayo we-endotoxin quantification.
Sikwabonelela nge-Bioendo KC Endotoxin Test Kit (Kinetic Chromogenic Assay) kunye ne-incubation microplate reader ELx808IU-SN, enokuqinisekisa ukuthembeka kunye nokusebenza kakuhle kovavanyo lwakho.
Ziziphi iimpawu zeuvavanyo lwe-kinetic chromogenic endotoxinukuvavanya i-endotoxins kwiisampuli?

Uvavanyo lovavanyo lwe-kinetic chromogenic endotoxin yenye indlela esetyenziselwa ukuvavanya i-endotoxins kwiisampulu.Ineempawu ezininzi:
1. Umlinganiselo weKinetic: Ngokufana ne-turbidimetric assay, i-kinetic chromogenic assay nayo ibandakanya umlinganiselo we-kinetic.Ixhomekeke kwimpendulo phakathi kwe-endotoxins kunye ne-chromogenic substrate ukuvelisa imveliso enemibala.Utshintsho kumandla wombala ekuhambeni kwexesha lubekwe esweni, luvumela ubungakanani bokuxinana kwe-endotoxin kwisampulu.
2. Uvakalelo oluphezulu: I-kinetic chromogenic assay inobucayi kwaye inokubona amanqanaba aphantsi e-endotoxins kwiisampuli.Inokulinganisa ngokuchanekileyo ugxininiso lwe-endotoxin, nokuba kumanqanaba aphantsi kakhulu, iqinisekisa ukufunyanwa okuthembekileyo kunye nobungakanani.
3. Uluhlu olubanzi oluguquguqukayo: Uvavanyo lunoluhlu olubanzi oluguquguqukayo, oluvumela umlinganiselo we-endotoxin yogxininiso kwi-spectrum ebanzi.Oku kuthetha ukuba inokuvavanya iisampulu ezinamanqanaba ahlukeneyo e-endotoxins, evumela ukuxinana okuphantsi kunye nokuphezulu ngaphandle kwesidingo sokuxutywa kwesampulu okanye ukugxila.
4. Iziphumo ezikhawulezayo: I-kinetic chromogenic assay inikeza iziphumo ezikhawulezayo xa kuthelekiswa neendlela zendabuko.Ngokuqhelekileyo inexesha elifutshane lokuvavanya, livumela uvavanyo olukhawulezayo kunye nohlalutyo lweesampulu.Ukukhula kombala kunokujongwa ngexesha lokwenyani, kwaye iziphumo zihlala zifumaneka kwimizuzu embalwa ukuya kwiiyure ezimbalwa, kuxhomekeke kwikhithi yovavanyo kunye nezixhobo ezisetyenzisiweyo.
5. Ukuzenzekelayo kunye nokulinganisa: Uvavanyo lunokwenziwa kusetyenziswa iinkqubo ezizenzekelayo, ezifana nabafundi be-microplate okanye
i-endotoxin-specific analyzers.Oku kuvumela uvavanyo oluphezulu kunye nokuqinisekisa imilinganiselo ehambelanayo kunye nemigangatho, ukunciphisa impazamo yabantu kunye nokwandisa ukusebenza kakuhle.
6. Ukuhambelana neentlobo ezahlukeneyo zeesampuli: I-kinetic chromogenic assay ihambelana noluhlu olubanzi lweentlobo zeesampuli, kubandakanywa amayeza, izixhobo zonyango, i-biologics, kunye neesampuli zamanzi.Yindlela eguquguqukayo enokuthi isetyenziswe kumashishini ahlukeneyo kunye nezicelo apho uvavanyo lwe-endotoxin lufuneka.

 

Lilonke, uvavanyo lwe-kinetic chromogenic endotoxin assay lubonelela ngendlela enovakalelo, ekhawulezayo, kunye nethembekileyo yokubhaqa kunye nokulinganisa
i-endotoxins kwiisampuli.Isetyenziswa kakhulu kumayeza, kwi-biotechnology, nakumashishini okhathalelo lwempilo ukulawula umgangatho kunye nokhuseleko.
iinjongo zovavanyo.