Uvavanyo loVavanyo lwe-Endotoxin yiLyophilized Amebocyte Lysate (LAL Reagent)

Uvavanyo loVavanyo lwe-Endotoxin yiLyophilized Amebocyte Lysate (LAL Reagent)

LAL Reagents: I-Lyophilized amebocyte lysate (LAL) isicatshulwa esinamanzi seeseli zegazi (i-amebocytes) ukusuka kwi-Atlantic horseshoe crab.
I-TAL Reagents: i-reagent ye-TAL yincindi ye-aqueous yeeseli zegazi ukusuka kwi-Tachypleus tridentatus.
Okwangoku, eyona mveliso iphambili ye-LAL/TAL reagents ise-United States nase China.

Uvavanyo lovavanyo lwe-clot endotoxin ye-gel, le ndlela iseyeyona nkqubo iphambili kwihlabathi liphela, ukuze kuhlangatyezwane nemfuneko yokhuseleko lwamayeza enaliti yoMntu.
NGOKU iBioendo ivelisa kwaye ibonelela ngehlwili lejeli i-LAL reagent equka iiampule zeglasi enye yovavanyo kunye neengqayi ezininzi zovavanyo.
https://www.bioendo.com/gel-clot-endotoxin-assay/ G01, GS44, G02, G17 kunye ne-G52
Esi sisisombululo esinoqoqosho sokufunyanwa kwe-endotoxins esemgangathweni kuvavanyo lwechiza.Ngokukodwa kwiziyobisi ezitofwayo okanye iziyobisi zabazali zokuvavanya i-endotoxins kwi-WFI, i-API okanye iimveliso zeziyobisi ezigqityiweyo.Uvavanyo lovavanyo lwe-Endotoxin lubango oluphezulu lwezenzo zokusebenza, lufuna umqhubi onobuchule ukujongana novavanyo lwehlwili lejeli ukuze kuqinisekiswe iziphumo ezichanekileyo.

Isisombululo esipheleleyo seG52Uvavanyo lovavanyo lwe-Endotoxin:
LAL Reagent
Ukulawula i-Endotoxin esemgangathweni
BET Amanzi
Iingcebiso ze-endotoxin-free pipette
Iityhubhu zeglasi ezingenayo i-endotoxin, kubandakanywa nokusebenza kodilution kunye neetyhubhu zokusabela.
Isixhobo sokufukamela, ukucebisa ngebhafu yamanzi okanye i-incubator yobushushu eyomileyo.Zonke izixhobo zokufukamela zifuna ukuchaneka kobushushu.
Zonke izinto ezisetyenziselwa ukuchukumisa i-LAL assay edibana nomgangatho we-endotoxin-free level ” <0.005EU/ml” .
Indawo yovavanyo iya kulungela ukubhaqwa kwe-endotoxin.

Naka u :
Izinto ezisetyenziswa kwiplastiki ezifana neengcebiso zepipette okanye iipleyiti zemultiwell zisetyenziswa ngokubanzi kuvavanyo lwe-endotoxin.I-Pharmacopoeias ehambelanayo (USP / CP) idinga ukuba naziphi na izinto ezisetyenziswayo zeplastiki kunye neetyhubhu zeglasi kufuneka zingabikho kwi-endotoxin ebonakalayo kwaye ibaluleke ngokulinganayo kodwa kaninzi ingaqwalaselwanga, kufuneka ibe ngumgangatho okhululekileyo kwizinto eziphazamisayo.

Ulusebenzisa njani uvavanyo lwe-endotoxin ukufumana i-endotoxins kwisampulu?
Okokuqala, uvavanyo lokuqinisekisa ubuzaza be-lysate kufuneka lwenziwe.Ukuqinisekisa uvakalelo kuyafana kumanqaku eleyibhile.
Uhlalutyo lwesampulu, yenza iimvavanyo zovavanyo lwe-endotoxin kwangaphambili.
Ukusebenzisa uvavanyo olupheleleyo lwe-endotoxin "yovavanyo lokuphazamiseka".
Ukusebenzisa uvavanyo olulinganiselweyo lokufumana ukuba linjani inqanaba le-endotoxins kwisampulu.

Xa usebenza uvavanyo lokuqinisekisa okubhalwe uvakalelo lwe-lysate, isiphumo asiqhelekanga, i-2 Lamada havenot gel formation?
Jonga ukuba indlela yokulungiselela yomgangatho we-endotoxin yolawulo lwebhaktiriya ichanekile.
Ukuxuba i-Vortex kuyafuneka kwi-diluent nganye (jonga incwadi yemiyalelo okanye ebizwa ngokuba yi-endotoxin yolawulo olusemgangathweni kwiinkcukacha).
Ukujonga ukuba i-reagent ye-lysate ihambelana nomgangatho wolawulo we-endotoxin.

Kunconywa ukusebenzisa ukuhlamba kwamanzi okanye i-incubator yokushisa eyomileyo kwinkqubo yokushisa rhoqo ye-lysate reagent (umzekelo, i-incubator okanye ibhokisi elomileyo ayisebenzi).
Ngethuba lokufukamela, indawo yamanzi kwindawo yokuhlambela amanzi e-static kwaye ezinzileyo, zonke ii-vibrations ziyagwenywa.
Nika ingqalelo ekhethekileyo kwindawo yokuhlambela amanzi ukucima impompo yokuhamba kwamanzi.

Ukujonga isiphumo, ngobunono khupha ityhubhu yovavanyo okanye i-ampoule yeglasi kwi-thermostat, ujike kancinci i-180 degrees,
ukubunjwa kwe-gel kwi-tube akuyi kuphazamiseka, kwaye ayiyi kuphazamiseka, umphumo wabhalwa ngumqondiso "+";
akukho kwakheka kwejeli kwenziwa okanye ihlwili lejeli alinakugcinwa licocekile nangona lenziwe.
Isiliphu sodonga sibi, umphumo wabhalwa ngumqondiso "-".
Ijeli ekhawulezayo yovavanyo lwehlwili ye-endotoxin Ikhithi yeyendlela yovavanyo yehlwili yejeli ye-endotoxin.
Kuvavanyo olukhawulezayo lwe-clot endotoxin yejeli, isampulu yolawulo olulungileyo ayivelisi ijeli?
Okokuqala, sebenzisa amanzi okuvavanya i-endotoxin ye-bacterial ukuqinisekisa i-reagents ye-lysate, uqinisekise ukuba abaqhubi kunye nokusingqongileyo bahlangabezane nemimiselo;

Ukuba ikiti ifanelekile, ngoko ulawulo olulungileyo lwesampulu aluyiyo i-gel ukwakheka ngenxa yempembelelo yokuthintela isampuli, kwaye isampuli kufuneka iqhutywe kwaye iphathwe.
Eyona ndlela iqhelekileyo yokusetyenzwa kwesampulu kukuhlanjululwa.