I-Endotoxin-Free Water: Ukudlala indima ebalulekileyo kwii-Endotoxin Test Assays
Intshayelelo:
Uvavanyo lwe-Endotoxin licandelo elibalulekileyo kumashishini ahlukeneyo, kubandakanya amayeza, izixhobo zonyango, kunye ne-biotechnology.Ukufunyanwa okuchanekileyo nokuthembekileyo kwe-endotoxins kubalulekile ukuqinisekisa ukhuseleko lwemveliso kunye nokuthotyelwa kwemigangatho yolawulo.Enye imfuneko esisiseko yokwenza uvavanyo lwe-endotoxin kukusetyenziswa kwamanzi angenayo i-endotoxin.Kule nqaku, siza kuhlolisisa ukubaluleka kwamanzi angenayo i-endotoxin, indima yayo ekwenzeni i-Lyophilized Amebocyte Lysate (LAL) iimvavanyo ze-endotoxin, kunye nokubaluleka kokusebenzisa amanzi angenayo i-endotoxin kwi-Bacterial Endotoxin Test (BET).
Ukuqonda i-Endotoxins:
Ii-Endotoxins zi-lipopolysaccharides (LPS) ezifumaneka kwinwebu yangaphandle ye-Gram-negative bacteria.Bangabalamli abanamandla bokuvuvukala kwaye banokubangela iziphumo ezibi kakhulu xa zikhona kwiimveliso zamachiza okanye izixhobo zonyango.Ngenxa yamandla abo okubangela ukuphendulwa kwe-pyrogenic, ukufumanisa ngokuchanekileyo kunye nobungakanani be-endotoxins kubalulekile.
Uvavanyo lwe-LAL Endotoxin:
Eyona ndlela eyaziwayo yovavanyo lwe-endotoxin luvavanyo lwe-LAL, olusebenzisa igazi loononkala.I-Limulus polyphemus kunye neTachypleus tridentatus.I-reagent ye-Lyophilized Amebocyte Lysate (LAL) itsalwa kwiiseli zegazi zoononkala, equlethe iprotheni ehlwithayo eyenziwa xa kukho i-endotoxins.
Indima yeI-Endotoxin-Free WaterkuVavanyo lwe-LAL:
Amanzi yinxalenye ephambili ekulungiseleleni i-reagent kunye namanyathelo okuxutywa kovavanyo lwe-LAL.Nangona kunjalo, nokuba ulandele amanani e-endotoxins akhoyo kumanzi ompompo rhoqo anokuphazamisana nokuchaneka kunye novakalelo lovavanyo.Ukoyisa lo mngeni, amanzi angenayo i-endotoxin kufuneka asetyenziswe kuyo yonke inkqubo yovavanyo.
Amanzi angenayo i-Endotoxin adlala indima ebalulekileyo ekuqinisekiseni ukuba iirejenti ezisetyenziswa kuvavanyo lwe-LAL azingcoliswanga yi-endotoxins.Ngaphaya koko, ikhusela iziphumo ezingezizo zobuxoki okanye ezingezizo ezingezizo, ngaloo ndlela ibonelela ngobungakanani be-endotoxin ethembekileyo nechanekileyo.
Ukukhetha Amanzi aLungileyo kuVavanyo lwe-LAL:
Ukufumana amanzi angenayo i-endotoxin, iindlela ezininzi zokucoca zinokusetyenziswa.I-Deionization, i-distillation, kunye ne-osmosis eguquguqukayo ziindlela eziqhelekileyo ezisetyenziswayo zokunciphisa ubukho be-endotoxins emanzini.Obu buchule bususa ukungcola okwahlukeneyo, kubandakanywa i-endotoxins evela kwiibhaktheriya.
Ukongeza, kubalulekile ukuqinisekisa ukuba izitya ezisetyenziselwa ukugcina, ukuqokelela, kunye nokuhambisa amanzi angenayo i-endotoxin ziqinisekisiwe ngokufanelekileyo kwaye azinasulelo kwi-endotoxin.Oku kubandakanya ukusebenzisa i-endotoxin-free tubes, iibhotile, kunye nezihlungi ngexesha lenkqubo.
Ukubaluleka kwaManzi e-BET:
KwiUvavanyo lwe-Endotoxin yebhaktiriya (BET), amanzi angenayo i-endotoxin, eyaziwa ngokuba ngamanzi e-BET, asetyenziswa njengolawulo olubi ukuqinisekisa uvakalelo kunye neenkcukacha zovavanyo lwe-LAL.Amanzi e-BET kufuneka aqulathe inqanaba elingabonakaliyo le-endotoxins, eqinisekisa ukuba nawuphi na umsebenzi we-endotoxin onokulinganiswa uthathwa kuphela kwisampulu evavanyiweyo.
Ukusetyenziswa kwamanzi e-BET kuvavanyo lwe-endotoxin lusebenza njengolawulo olubalulekileyo lokuqinisekisa ukusebenza kwe-LAL reagents, inkqubo yovavanyo kunye nezixhobo.Eli nyathelo lokuqinisekisa libalulekile ukuvavanya ngokuchanekileyo ubukho kunye nokuxinana kwee-endotoxins kwisampulu evavanyiweyo.
Isiphelo:
Amanzi angenayo i-Endotoxin adlala indima ebalulekileyo ekubhaqweni ngokuchanekileyo nangokuthembekileyo kwe-endotoxins kumashishini ahlukeneyo.Kuvavanyo lwe-LAL endotoxin, iqinisekisa ukuba iirejenti ezisetyenzisiweyo azingcolisekanga, zibonelela ngobungakanani obuchanekileyo.Kwi-BET, amanzi angenayo i-endotoxin asebenza njengolawulo, eqinisekisa uvakalelo lovavanyo lwe-LAL.Ngokubambelela kwiindlela zokucoca ezingqongqo kunye nokusebenzisa izikhongozeli eziqinisekisiweyo, amandla eziphumo zobuxoki kunye neempazamo zinokuncitshiswa kakhulu.
Njengoko ukubaluleka kovavanyo lwe-endotoxin kuqhubeka ukukhula, indima yamanzi angenayo i-endotoxin ibaluleke ngakumbi.Ukusebenzisa iindlela ezithembekileyo zokucocwa kwamanzi kunye nokubandakanya ezona ndlela zibalaseleyo kwinkqubo yovavanyo kuya kuqinisekisa ukhuseleko kunye nokuthotyelwa kweemveliso zamayeza, izixhobo zonyango, kunye nezinye izinto ezibuthathaka kwi-endotoxin.
Ixesha lokuposa: Nov-29-2023
