I-Recombinant Factor C (rFC) uvavanyoyindlela esetyenziswa ngokubanzi ekufumaneni i-endotoxins yebhaktheriya, eyaziwa ngokuba yi-lipopolysaccharides (LPS), i-Endotoxins yinxalenye ye-membrane yangaphandle ye-Gram-negative bacteria enokubangela impendulo enamandla yokuvuvukala kwizilwanyana, kuquka nabantu.Uvavanyo lwe-rFC lusekwe ekusetyenzisweni kohlobo lobunjineli bemfuza ye-Factor C, i-enzyme efumaneka ngokwemvelo kwigazi le-horseshoe crab kwaye ibandakanyeka kwi-clotting pathway.Kwi-rFC assay, i-recombinant Factor C isetyenziselwa ukufumanisa ubukho be-endotoxins ngokulinganisa Ngokulinganisa umxholo we-substrates ecandiweyo phambi kwe-endotoxin.Xa kuthelekiswa neendlela zemveli zokufunyanwa kwe-endotoxin, njenge-Limulus Amebocyte Lysate (LAL) assay esebenzisa igazi le-horseshoe crab, i-rFC assay ithathwa njengento eqhelekileyo kwaye iphinda iphinde iphinde iphinde iphinde iphinde iphinde iphinde iphinde iphinde iphinde iphinde isetyenziswe, njengoko ingathembeli ekusebenziseni i-reagents ephuma kwizilwanyana.Uvavanyo lwe-rFC lukwanobuhlobo ngakumbi kokusingqongileyo kwaye luzinzile, njengoko lunciphisa imfuno yokuqokelela kunye nokusetyenziswa koononkala beehorseshoe ekubhaqweni kwe-endotoxin.
Uvavanyo lwe-rFC luvunyiwe ngabasemagunyeni abalawulayo, njenge-United States Pharmacopeia (USP) , i-European Pharmacopoeia (EP) kunye ne-Chinese Pharmacopoeia (CP) yokusetyenziswa kuvavanyo lokulawula umgangatho wamachiza kunye nezixhobo zonyango.
Uncedo lwe-recombinant factor c assay
Uvavanyo lwe-Recombinant Factor C (rFC) lubonelela ngeenzuzo ezininzi kwiindlela zemveli zokubona i-endotoxins, ezifana ne-Limulus Amebocyte Lysate (LAL) assay.Ezinye zezibonelelo zovavanyo lwe-rFC ziquka:
1. Umgangatho: I-rFC assay yiteknoloji ye-DNA ephinda iphinda isebenzise iprotheni enye, echazwe njenge-reagent yokufumanisa.Oku kwenza ukuba uvavanyo lube semgangathweni kwaye lungabikho ukuguquguquka xa kuthelekiswa novavanyo lwe-LAL, oluxhomekeke ekusebenziseni umxube onzima weeprotheni ezikhutshwe kwigazi le-horseshoe crab.
2. Ukuveliswa kwakhona: Uvavanyo lwe-rFC lunamanqanaba aphezulu okuphindaphinda, njengoko lisebenzisa iprotheni enye, echazwe njenge-reagent yokufumanisa.Oku kuvumela iziphumo ezingaguqukiyo, nakwiibhetshi ezahlukeneyo kunye neereagents ezininzi.
3. Ukusetyenziswa kwezilwanyana okucuthiweyo: Uvavanyo lwe-rFC yindlela ehambelana nokusingqongileyo kunye nezinzileyo yokubona i-endotoxins, njengoko ayifuni ukusetyenziswa kwezilwanyana eziphilayo okanye ezibingelelweyo, ezifana noononkala beehashe.
4. Indleko eyongayo: Uvavanyo lwe-rFC luxabisa kakhulu kunovavanyo lwe-LAL, ngenxa yokuncitshiswa kwemfuno yezilwanyana eziphilayo kunye nemo yovavanyo olusemgangathweni.
5. Uzinzo: Uvavanyo lwe-rFC luqinile kwaye lunokusetyenziswa kwiintlobo ezininzi zezicelo, kubandakanywa ukuhlolwa kokulawulwa komgangatho wamachiza, izixhobo zonyango, kunye nezinye iimveliso ezinokuthi ziqulethe i-endotoxins.
6. Imvume yokulawula: Uvavanyo lwe-rFC luvunyiwe ngabasemagunyeni abalawulayo njenge-United States Pharmacopeia (USP) , i-European Pharmacopoeia (EP) kunye ne-Chinese Pharmacopoeia (CP) yokusetyenziswa kuvavanyo lokulawula umgangatho wamachiza kunye nezixhobo zonyango.Oku kunika inqanaba eliphezulu lokuzithemba ekuthembekeni kunye nokuchaneka kovavanyo.
Ukuhlangabezana nezidingo ezahlukeneyo, i-Bioendo ikwavelisa kwaye ibonelela ngendlela yemveli ye-gel clot endotoxin test assay kit, ijeli ekhawulezayo ye-clot assay kit, ikiti yovavanyo lwe-endotoxin yobungakanani kubandakanya "ikiti yovavanyo lwe-kinetic turbidimetric endotoxinkwayeikiti yovavanyo lwe-kinetic chromogenic endotoxin” .
Ixesha lokuposa: Feb-19-2023
