Izinto ezisetyenziswayo ezingena-pyrogen ziyinto etyiwayo ngaphandle kwe-endotoxin yangaphandle, kubandakanywa iingcebiso zepipette ezingena-pyrogen (ibhokisi yetip), iityhubhu zokuvavanya ezingenazo ipyrogen okanye ezibizwa ngokuba yi-endotoxin yeetyhubhu zeglasi ezisimahla, ii-ampoules zeglasi ezingena-pyrogen, i-endotoxin-free 96-well-well microplates, kunye ne-endotoxin- amanzi akhululekile (ukusetyenziswa kwamanzi e-depyrogenated kuvavanyo lwe-endotoxin ye-bacterial), i-endotoxin ye-buffer yamahhala kunye njl. Phakathi kwabo, amanzi e-bacterial endotoxintest nge-gel clot method kunye novavanyo lwe-endotoxin yovavanyo lwe-endotoxin kuzo zonke i-Pharmacopoeia editions (USP, EP, BP, JP) kunye neChina Pharmaceutica) .Ibhekisa kumanzi acwebileyo okutofwa ngomxholo we-endotoxin ngaphantsi kwe-0.015EU/ml.Ngoku inguqulelo yamva nje ye-Pharmacopoeia, amanzi e-BET angaphantsi kwe-0.005EU/ml.Nokuba umgangatho ophezulu ungaphantsi kwe-0.001EU / ml iyakwazi ukuveliswa kunye nokunikezelwa yi-Bioendo.
Amagama aphambili ahambelanayo e-endotoxins, kunye ne-endotoxin esetyenziswa simahla, ipyrogen kunye nemithombo yobushushu zikhonsepthi ezimbini ezahlukeneyo ngokupheleleyo:IProjeni: Ikwabizwa ngokuba yipyrogen okanye exothermic factor.izinto ezinokubangela ukwanda kobushushu bomzimba.Umthombo wobushushu: Into ekhupha ubushushu.Okufana nokutshisa imatshisi, amalahle, njl.Okubizwa ngokuba yi "non-pyrogenic consumables" kunye "nempendulo ye-pyrogenic" yabanye abavelisi kunye nabarhwebi ngokwenene ngamagama angenamsebenzi kwaye alahlekisayo.Ezona zichanekileyo kufuneka zibe "I-Pyrogen Free" kunye ne "Pyrogen Response".
Kutheni le nto izinto ezisetyenziswayo zasimahla zePyrogen ziyimfuneko kuvavanyo lovavanyo lwe-endotoxin, zombini ii-gel clot endotoxin test assay kunye ne-endotoxin test assay?
Ewe, izinto ezisetyenziswayo ezingena-pyrogen zibalulekile ekwenzeni uvavanyo lwe-endotoxin ngokuchanekileyo nangokuthembeka.Ubukho be-pyrogens, okuyizinto ezibangela umkhuhlane ovame ukuvela kwi-endotoxins ye-bacterial, inokuphazamisa iziphumo zovavanyo kwaye ikhokelele ekufundweni kobuxoki.Uvavanyo lwe-endotoxin, oluqhelekileyo olubizwa ngokuba luvavanyo lwe-Limulus amebocyte lysate (LAL) okanye olubizwa ngokuba yi-Lyophilized amebocyte lysate (LAL) uvavanyo, lusetyenziselwa ukufumanisa kunye nokulinganisa ubukho be-endotoxins yebhaktiriya kumayeza, izixhobo zonyango, kunye nezinye iimveliso.Uvavanyo lwe-LAL luxhomekeke kwimpendulo phakathi kwe-LAL reagent kunye ne-endotoxins ukuvelisa i-clotting okanye impendulo ye-chromogenic.Ukuqinisekisa iziphumo ezichanekileyo, kubalulekile ukusebenzisa izinto ezisetyenziswayo ezingenazo ii-pyrogens.I-Pyrogens inokungcolisa izinto ezahlukeneyo zebhubhoratri, kubandakanywaiglasi, iingcebiso zepipette, iityhubhu, kunye nezikhongozeli zeesampulu.Ukuba izinto ezisetyenziswayo ezingcoliswe yipyrogen zidibana ne-reagent ye-LAL okanye iisampulu zovavanyo, zinokubangela ukusabela okungeyonyani, okukhokelela kwizigqibo ezigwenxa malunga nobukho okanye ukuxinana kwee-endotoxins.Izinto ezisetyenziswayo ezingenaPyrogen zenziwe kwaye zivavanywe ngokukodwa ukunciphisa okanye ukuphelisa ubukho be-pyrogens.Bangena kwiinkqubo ezingqongqo zolawulo lomgangatho ukuqinisekisa ukuba bayahlangabezana nemigangatho efunekayo yovavanyo lwe-endotoxin.Ukusebenzisa ezi zinto zokusetyenziswa ezikhethekileyo kunceda ukugcina ingqibelelo kunye nokuchaneka kovavanyo lovavanyo lwe-endotoxin, ukuqinisekisa iziphumo ezithembekileyo kunye nokukhuthaza ukhuseleko lwesigulane kwi-pharmaceutical and biomedical applications.
Ixesha lokuposa: Nov-07-2022
