Yintoni i-Endotoxin

I-Endotoxins ziimolekyuli ezincinci ze-hydrophobic lipopolysaccharides (LPS) eziphuma kwibhaktiriya ezifumaneka kwi-membrane yeseli engaphandle yebhaktiriya yegram-negative.I-Endotoxins iqulethe i-core polysaccharide chain, i-O-specific polysaccharide side chain (O-antigen) kunye ne-lipid compenent, i-Lipid A, ejongene nemiphumo eyityhefu.Iibhaktheriya zichitha i-endotoxin ngezixa ezikhulu ekufeni kweeseli naxa zikhula kwaye zisahlula.IEscherichia coli enye iqulethe malunga nezigidi ezi-2 zeeathom ze-LPS kwiseli nganye.

I-Endotoxin inokosulela ngokulula iilebhu, kwaye ubukho bayo bunokubonelela ngokubonakalayo kokubini kwi-vitro nakwimifuniselo ye-vivo.Kwaye kwiimveliso zabazali, iimveliso zabazali ezingcoliswe yi-endotoxins ezibandakanya i-LPS zinokukhokelela ekuphuhliseni umkhuhlane, ukungeniswa kwempendulo yokuvuvukala, ukothuka, ukusilela kwamalungu kunye nokufa ebantwini.Kwiimveliso ze-dialysis, i-LPS inokudluliselwa kwi-membrane enobungakanani obukhulu be-pore ngokuhluzwa ngasemva ukusuka kwi-dialysis fluid ukuya egazini, iingxaki ezivuthayo zinokubangelwa ngokufanelekileyo.

I-Endotoxin ifunyenwe yiLyophilized Amebocyte Lysate (TAL).I-Bioendo inikezelwe kuphando, ukuphuhlisa nokuvelisa i-reagent ye-TAL iminyaka engaphezu kwamashumi amane.Iimveliso zethu zigubungela zonke iindlela ezisetyenzisiweyo ukukhangela i-endotoxin, obubuchule bejeli-clot, ubuchule be-turbidimetric, kunye nobuchule bechromogenic.