I-EtEraser™ HP Endotoxin Removal Kit

I-EtEraser™ SE Ikhithi yokuSusa i-Endotoxin

1. Ulwazi lwemveliso

Siyingcali yovavanyo lwe-endotoxin kunye ne-endotoxinremoval.Sinikezela ngezisombululo ezipheleleyo ezigubungela lonke uluhlu lwe-endotoxincontrol, endotoxin esweni kunye novavanyo.Iimveliso zethu ziquka ukusetyenziswa kophando lwaselabhoratriikhithi yokususa i-endotoxins kwaye inokunyuswa ukuya kwiikholamu zesikali semveliso.I-Lipopolysaccharide (LPS) yi-endotoxin yebhaktiriya kwaye iyinxalenye enkulu yeendonga zeeseli zentsholongwane yegram-negative.I-Recombinantprotein esuka kwi-E.coliusally iqulethe inqanaba eliphezulu leendotoxins.Ukususwa kwezi endotoxins kubaluleke kakhulu kwiinkqubo ezisezantsi.

EtEraser HP Endotoxin Removal Kitziyilelwe ukususa ungcoliseko lwe-endotoxin kwizicombululo ezinamanzi.Iikiti ziqulethe i-endotoxin yokususa i-resinbinds ukunciphisa amanqanaba e-endotoxin kwiisampuli zeprotheni nge-≥99% ngaphantsi kweeyure ze-2.

Le kiti yokususa i-endotoxin inokusetyenziswa kwiprotheyini, i-DNA/RNA, i-polysaccharide kunye nezinye iisampulu zebhayoloji.

2.Iimpawu zemveliso

◆ ukuzinza okuphezulu - akuchaphazeli umsebenzi wesampulu yezinto eziphilayo

◆ ukubuyiswa kweprotheyini ephezulu - > i-95% yokubuyisela iprotheni kwiisampuli zeprotheni

◆ ukususwa okuphezulu kokususa - susa> I-99% ye-endotoxin, inqanaba le-endotoxin kwisampuli ingaba ngaphantsi kwe-0.1 EU / mlafter endotoxin yokususa inkqubo

◆ uluhlu olubanzi lwesicelo - lunokusetyenziswa ekususweni kwe-endotoxin kwiiprotheni, iipeptides, izilwa-buhlungu, izitofu, i-polysaccharide kunye nezinye iisampulu zebhayoloji.

I-EtEraserHP Isixhobo sokususa i-endotoxin ePhakamileyo isebenzisa immobilized affinity ligandmodified PMB ukubopha nokususa i-endotoxin kwisisombululo esimanzi.I-modifiedPMB (i-polymyxin B) i-ligand yi-endotoxin ephezulu ebopha i-ligand.Ikholomu inezinga eliphezulu kakhulu lokubuyiswa kweeprotheyini ezingaphezu kwama-95%.Inqanaba le-Endotoxin kwisampulu linokuba ngaphantsi kwe-0.1 EU / ml emva kwenkqubo yokususa i-endotoxin.

Ikitin ibandakanya ikholamu yokususa i-endotoxin epakishwe ngaphambili eyi-1.5 ml, i-Equilibration Buffer, i-Regeneration Buffer kunye netyhubhu yokuqokelela engena-pyrogen kunye neengcebiso.Ikholamu inomthamo wokubopha ophezulu we> 2, 000, 000 EU / ml.Le mveliso inokuphinda isetyenziswe ukuya kumaxesha amahlanu ukuba ihlaziywe ngokufanelekileyo.I-affinity resin iyafumaneka i-inslurry kwaye inokunyuswa kwinkqubo ye-biopharmaceutical.