Ekusebenzeni kwei-bacterial endotoxin test assay, ukusetyenziswa kwamanzi angenayo i-endotoxin kunyanzelekile ukunqanda ukosuleleka.Ubukho be-endotoxins emanzini bunokukhokelela kwiziphumo ezingachanekanga kunye neziphumo zovavanyo eziphazamisekileyo.Kulapho i-Lyophilized Amebocyte Lysate (LAL) i-reagent yamanzi kunye ne-bacterial endotoxin test (BET) amanzi angena khona.La manzi ayilwe ngokukodwa abalulekile ekuqinisekiseni ukuthembeka kunye nokuchaneka kovavanyo lwe-endotoxin kumashishini ahlukeneyo, kubandakanywa amayeza, izixhobo zonyango, iilebhu zophando njalo njalo.
II-LAL yamanzi e-reagentngamanzi acocwe kakhulu alungiselelwe ngokukodwa ukusetyenziswa kuvavanyo lwe-LAL yee-endotoxins.La manzi aphantsi kwenkqubo yokwenziwa engqongqo ukuqinisekisa ukuba akanayo i-endotoxins, enokuphazamisana neziphumo zovavanyo.Ukungabikho kwee-endotoxins kumanzi e-reagent ye-LAL kubalulekile ekuqinisekiseni ubuntununtunu kunye neenkcukacha ezithile zovavanyo lwe-LAL, kuyenza ibe lolona khetho lufanelekileyo lokubhaqwa kwe-endotoxin.
Ngokufanayo, amanzi e-BET nawo ayinxalenye ebalulekileyo kuvavanyo lovavanyo lwe-endotoxin yebhaktiriya.La manzi alungiselelwe ngokukodwa kwaye avavanywe ukuze kuqinisekiswe ukuba akhululekile kwi-endotoxins kunye nezinye izinto ezingcolileyo ezinokuchaphazela ukuchaneka kovavanyo.Ukusebenzisa amanzi e-BET kuvavanyo lovavanyo lwe-endotoxin kubalulekile ekufumaneni iziphumo ezinokwethenjelwa kunye nokuveliswa kwakhona, njengoko kuphelisa umngcipheko wezinto ezingalunganga okanye izinto ezingalunganga ezinokuthi zenzeke ngenxa yobukho be-endotoxins emanzini aqhelekileyo.
Ukubaluleka kokusebenzisa amanzi angenayo i-endotoxin kwi-endotoxin test assay ayikwazi ukugqithiswa.Ukuchaneka nokuthembeka kweziphumo zovavanyo kuxhomekeke kumgangatho wamanzi asetyenzisiweyo.Ubukho be-endotoxins emanzini bunokukhokelela ekufundweni kobuxoki, okunokuba nemiphumo emibi kumashishini apho uvavanyo lwe-endotoxin lubalulekile ekuqinisekiseni ukhuseleko kunye nokusebenza kakuhle kweemveliso.Ke ngoko, utyalo-mali kumanzi asebenzayo e-LAL okanye amanzi e-BET kubalulekile ukugcina ingqibelelo yenkqubo yovavanyo lwe-endotoxin kunye nokuqinisekisa umgangatho kunye nokhuseleko lweemveliso.
Ukuqukumbela, ukusetyenziswa kwamanzi angenayo i-endotoxin, njengamanzi e-reagent ye-LAL kunye namanzi e-BET, kubalulekile ekusebenzeni kovavanyo lovavanyo lwe-bacterial endotoxin.La manzi alungiselelwe ngokukodwa ayilelwe ukuphelisa umngcipheko wongcoliseko kunye nokuqinisekisa ukuchaneka nokuthembeka kovavanyo lwe-endotoxin.Ngokusebenzisa la manzi, amashishini anokwenza uvavanyo lwe-endotoxin ngokuzithemba ngaphandle koloyiko lweziphumo ezingachanekanga ngenxa yobukho be-endotoxins emanzini.Ekugqibeleni, ukusetyenziswa kwamanzi e-reagent ye-LAL kunye namanzi e-BET kubalulekile ekuphakamiseni imigangatho ephezulu yomgangatho kunye nokhuseleko kumashishini apho uvavanyo lwe-endotoxin lubaluleke kakhulu.
Xa usenza uvavanyo lwe-endotoxin yebhaktiriya, kubalulekile ukusebenzisa amanzi angenayo i-endotoxin ukuqinisekisa iziphumo ezichanekileyo nezithembekileyo.
Ii-endotoxins zizinto ezizinzile zobushushu kudonga lweseli yebhaktiriya e-gram-negative, kwaye zinokubangela umkhuhlane, ukothuka, kunye nokufa ebantwini nakwizilwanyana.
Ke ngoko, kubalulekile ukusebenzisa amanzi angenayo i-endotoxins xa usenza uvavanyo.
Kukho iindidi ezininzi zamanzi ezinokuthi zisetyenziswe kuvavanyo lovavanyo lwe-endotoxin yebhaktiriya, kubandakanywa amanzi e-reagent ye-LAL, i-TAL ye-reagent yamanzi, kunye namanzi anonyango lwe-depyrogenation.Nganye kwezi ntlobo zamanzi zenzelwe ukuqinisekisa ukuba i-endotoxins ayikho, ngaloo ndlela iqinisekisa ukuchaneka kweziphumo zovavanyo.
Amanzi e-reagent ye-LAL ngamanzi athe avavanywa ngokukodwa kwaye aqinisekiswa ukuba akanazo ii-endotoxins.La manzi aqhele ukusetyenziswa kwi-Lyophilized Amebocyte Lysate (LAL) assay, eyona ndlela ixhaphakileyo yokubona i-endotoxins.Ngokusebenzisa i-LAL yamanzi e-reagent ekuvavanyeni, abaphandi banokuqiniseka ukuba amanzi ngokwawo awafaki nxaxheba kuyo nayiphi na imiphumo yobuxoki okanye yobuxoki.
Ngokufanayo, amanzi asebenza nge-TAL ngamanzi avavanyiweyo ngokukodwa kwaye aqinisekiswa ukuba akhululekile kwi-endotoxins.La manzi asetyenziswa ngokuqhelekileyo kwi-assay ye-Tachypleus Amebocyte Lysate (TAL), enye indlela eqhelekileyo yokufumanisa i-endotoxins.Ngokusebenzisa i-TAL ye-reagent yamanzi ekuvavanyeni, abaphandi banokuqiniseka ukuba amanzi ngokwawo awafaki nxaxheba kuyo nayiphi na imiphumo yobuxoki okanye engalunganga.
Amanzi anonyango lwe-depyrogenation yenye inketho yokuqinisekisa ukuba amanzi asetyenziswe kuvavanyo lovavanyo lwe-endotoxin yebhaktiriya ayinayo i-endotoxins.Unyango lwe-Depyrogenation lubandakanya ukususwa okanye ukungasebenzi kwe-pyrogens, kuquka i-endotoxins, emanzini.Oku kunokufezekiswa ngeenkqubo ezifana nokuhluzwa, i-distillation, okanye unyango lwekhemikhali.Ngokusebenzisa amanzi ngonyango lwe-depyrogenation kwi-assay, abaphandi banokuqiniseka ukuba amanzi ngokwawo awafaki nxaxheba kuyo nayiphi na imiphumo yobuxoki okanye yobuxoki.
Ke, kutheni le nto kubaluleke kangaka ukusebenzisa amanzi angenayo i-endotoxin kuvavanyo lovavanyo lwe-endotoxin yebhaktiriya?Ubukho be-endotoxins emanzini asetyenziswe kwi-assay kunokukhokelela kwiziphumo ezingachanekanga, ezinokuthi zibe nefuthe elibi kuphando kunye nezicelo zeklinikhi.Ngokomzekelo, ukuba i-endotoxins ikhona emanzini, inokukhokelela kwiziphumo ezilungileyo zobuxoki, ezibonisa ubukho be-endotoxins xa zingekho ngokwenene.Oku kunokukhokelela kwinkxalabo engeyomfuneko kunye nokusetyenziswa kakubi kwemithombo yoncedo ukulungisa umba ongekho ngokwenene.
Ngakolunye uhlangothi, ukuba i-endotoxins ikhona emanzini kwaye ingabonakali, inokukhokelela kwiziphumo ezingezizo zobuxoki, ezibonisa ukuba i-endotoxins ayikho xa ikhona ngokwenene.Oku kunokukhokelela ekukhululweni kweemveliso ezingcolileyo, ukubeka impilo yabantu kunye nezilwanyana emngciphekweni.
Ukongeza kwimpembelelo enokubakho ekuchanekeni kweziphumo zovavanyo, ukusebenzisa amanzi angenayo i-endotoxin-free nako kunokuchaphazela ukusebenza kovavanyo ngokwalo.I-Endotoxins inokuphazamisa i-reagents kunye nezixhobo ezisetyenzisiweyo kwi-assay, ekhokelela kwiziphumo ezingathembekiyo okanye ezingahambelaniyo.Ngokusebenzisa amanzi angenayo i-endotoxin, abaphandi banokunciphisa le mingcipheko kwaye baqinisekise ukuba uvavanyo lwenziwa phantsi kweemeko ezithembekileyo.
Ekugqibeleni, ukuqinisekisa ukuba amanzi asetyenziswe kuvavanyo lovavanyo lwe-endotoxin yebhaktiriya ayinayo i-endotoxins ibalulekile ekugcineni imfezeko yeziphumo zovavanyo.Ingaba usebenzisa i-LAL yamanzi e-reagent, i-TAL yamanzi e-reagent, okanye amanzi anonyango lwe-depyrogenation, abaphandi banokuthatha amanyathelo asebenzayo ukuqinisekisa ukuba amanzi awafaki negalelo kuyo nayiphi na into engafanelekanga okanye engahambelaniyo kwiziphumo zovavanyo.Ngokwenza njalo, banokuba nentembelo ekunyanisekeni kweziphumo zabo kwaye banokwenza izigqibo ezisekelwe kwiziphumo zovavanyo.
Ixesha lokuposa: Jan-26-2024
