I-EtEraser™ SE Ikhithi yokuSusa i-Endotoxin
I-EtEraser™ SE Ikhithi yokuSusa i-Endotoxin
1. Ulwazi lwemveliso
I-Lipopolysaccharide (LPS) yi-endotoxin yebhaktiriya kunye neyona nto iphambili kwiindonga zeeseli zebhaktheriya yegramnegative.Iprotheyini ye-recombinant esuka kwi-E.coli idla ngokuba nenqanaba eliphezulu leendotoxins.Ukususwa kwezi endotoxins kubaluleke kakhulu kwiinkqubo ezisezantsi.
I-EtEraser SE Ikhithi yokususa i-Endotoxinziyilelwe ukususa ungcoliseko lwe-endotoxin kwizisombululo ezinamanzi.Iikhithi zineendotoxin yokususa i-resin ibophelela ukunciphisa amanqanaba e-endotoxin kwiisampulu zeprotheyini nge≥99% ngaphantsi kweeyure ezi-2.
Ikiti ibandakanya ikholamu yokususa i-endotoxin epakishwe ngaphambili, i-1.5 ml, i-Equilibration Buffer, i-Regeneration Buffer kunye ne-pyrogen-freecollection tube kunye neengcebiso.Ikholamu inomthamo ophezulu wokubopha we> 2,000, 000 EU / ml.Le mveliso ingaphinda isetyenziswe ukuya kuthi ga kwizihlandlo ezihlanu ukuba yenziwe ngokutsha ngokufanelekileyo.I-affinity resin iyafumaneka kwi-slurry kwaye inokuba yinkqubo ye-biopharmaceutical ye-up-scalingin.
I-EtEraser SE Ikhithi yokususa i-Endotoxinisebenzisa ibakala lokutya elilungisiweyoε-poly-L-lysinei-ashigh affinity ligand ye-endotoxins.I-resin ngamaso e-agarose azinzile kwaye ayinayo impembelelo ye-cytotoxic kumzimba womntu.Emva kokucoca, akuyi kubakho ntsalela enetyhefu eseleyo kwiisampuli.Okuikhithi yokususa i-endotoxininokusetyenziswa kumachiza eprotheyini, amayeza okugonya, amajoni omzimba, i-DNA/RNA, i-polysaccharide kunye nezinye iisampulu zebhayoloji.
2. Iimpawu zemveliso
• uzinzo oluphezulu - aluchaphazeli umsebenzi wesampulu yebhayoloji
• umthamo omkhulu wokudibanisa —>2000000 EU/ml
• non-toxic —–> Non-toxic ukutya grade ligands, akukho cytotoxiceffects poly lysine
• ukubuyiswa kweeprotheyini eziphezulu — > 95% yeprotheyini yokubuyiswa kweeprotheyini
• ukusebenza kakuhle kokususa - susa> 99% endotoxin, inqanaba le-endotoxin kwisampulu linokuba ngaphantsi kwe-0.1 EU / ml emva kwenkqubo yokususa i-endotoxin
• Uluhlu olubanzi lwesicelo - lunokusetyenziswa ekususeni i-endotoxin kwiiproteni, iipeptides, izilwa-buhlungu, izitofu, i-polysaccharide kunye nezinye iisampuli zebhayoloji.